2
votes
3answers
33 views
If I have 4 sequence runs, 2 in each direction, 1 bp is different, on each, should I resequence?
I have a plate of colonies to sequence. I pick 2 colonies and sequence each in Fwd and Rev directions. I get back a single bp difference between the 2 strands. 2 bp have an T, …
-1
votes
3answers
88 views
State of computational genomics
The other day on facebook, I posted this link and some of my friend started discussing about this article.
It's "Watson Meets Moore" as Ion Torrent founder Jonatha Rothberg intro …
3
votes
1answer
54 views
k-mer based sequencing contamination detection
In a plant genome project I got a draft assembly (> 500Mbp, >500k contigs).
A number of contigs is no doubt bacterial in origin.
There are at least 3 peaks when it comes to GC c …
1
vote
2answers
21 views
How to set SHRiMP parameters for best sensitivity with 35bp colorspace data?
Hi,
I have 35bp Solid colorspace sequencing data, and the actual sequences to be mapped are 20-25bp after removing the linker sequence.
I hope to find all the hits allowing no mo …
